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THE EFFECTS OF TRITIATED THYMIDINE INCORPORATION ON SECONDARY ROOT PRODUCTION BY PISUM SATIVUM
Author(s) -
Hummon Margaret R.
Publication year - 1962
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1962.tb15044.x
Subject(s) - pisum , sativum , biology , thymidine , meristem , pericycle , cytoplasm , plant cell , tritium , tritiated water , xylem , botany , root hair , biochemistry , dna , shoot , physics , arabidopsis , gene , mutant , nuclear physics
H ummon , M argaret R. (Montana State U., Missoula.) The effects of tritiated thymidine incorporation on secondary root production by Pisum sativum. Amer. Jour. Bot. 49(10): 1038–1046. Illus. 1962.—Most studies of effects of radiation on plants have involved a general exposure of all the cells and tissues of an organ or entire plant. Tritiated thymidine offers a tool for selective irradiation of the nucleus with little effect on the cytoplasm of a cell. Furthermore, differential incorporation due to variation in the pattern of DNA synthesis permits selective irradiation of cells and tissues. In this study, developing primary roots of Pisum sativum were submerged for brief periods in a solution of tritiated thymidine. This resulted in an alteration of the lateral root pattern. In the area corresponding to the region of elongation during treatment, subsequent lateral root production was suppressed. This correlated with the portion of the root in which there was incorporation of tritiated thymidine into a high percentage of pericycle nuclei. Abnormal development of vascular tissues also occurred, with evidence of altered polarity in the xylem. Although incorporation also occurred in the apical meristem, the latter was not affected at this level of exposure. Thus, differential sensitivity as well as differential incorporation may have been involved in producing the temporary alteration of lateral root production.