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HISTOCHEMICAL AND AUTORADIOGRAPHIC STUDIES OF FLORAL INDUCTION IN CHENOPODIUM ALBUM
Author(s) -
Gifford Ernest M.,
Tepper Herbert B.
Publication year - 1962
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1962.tb15000.x
Subject(s) - biology , apex (geometry) , primordium , botany , rna , inflorescence , chenopodium , rosette (schizont appearance) , biochemistry , weed , gene , immunology
G ifford , E rnest M., J r. , and H erbert B. T epper . (U. California, Davis.) Histochemical and autoradiographic studies of floral induction in Chenopodium album. Amer. Jour. Bot. 49(7): 706–714. Illus. 1962.— Chenopodium album was induced to flower using short‐day photoperiods. Changes in the chondriome, starch, total protein, ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and histone distribution in cells of vegetative and inflorescence shoot apices were studied. The distal cells of the vegetative apex (especially the axial tunica cells) possess larger nucleoli and vacuoles, less granular mitochondria, and more differentiated plastids than do other cells of the apex; the distal cells stain lightly with dyes that indicate the presence of DNA and histone. RNA is distributed relatively uniformly in the shoot apex; the cells at sites of leaf initiation and young leaf primordia contain slightly higher concentrations of RNA than the axial cells of the shoot apex. Protein is uniformly distributed throughout the vegetative as well as the inflorescence apex. Upon induction, the chemical and morphological differences between cells in the shoot apex gradually disappear. RNA concentration of cells in the apex increases, reaching a maximum after 4 inductive cycles. Protein concentration of cells also increases, but this increase lags behind that of RNA.