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THE NUTRITION OF CERTAIN SPECIES OF THE GREEN ALGA OEDOGONIUM
Author(s) -
Machlis Leonard
Publication year - 1962
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1962.tb14925.x
Subject(s) - biology , axenic , axenic culture , botany , fractionation , chromatography , chemistry , bacteria , genetics
M achlis , L eonard . (U. California, Berkeley.) The nutrition of certain species of the green alga Oedogonium. Amer. Jour. Bot. 49 (2) : 171–177. Illus. 1962.—Evidence is presented showing that O. cardiacum and O. geniculatum require vitamin B 12 and an unknown growth factor released from soil into water during autoclaving. The unknown growth factor is shown not to be a number of things, to be retained on anion exchange columns, and to be partially purified by fractionation on the polydextran, Sephadex 50. It appears to be an organic substance with acidic properties. Isolates of Oedogonium presently in unialgal and axenic culture are listed and the methods used to obtain the axenic cultures and to grow them for nutritional studies are described.