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ISOLATION, GROWTH, AND RESPIRATION OF A THERMOPHILIC BLUE‐GREEN ALGA
Author(s) -
Holton Raymond W.
Publication year - 1962
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1962.tb11738.x
Subject(s) - aeration , biology , respiration , laboratory flask , thermophile , incubation , botany , algae , bacteria , cyanobacteria , dry weight , bacterial growth , distilled water , blue green algae , horticulture , ecology , chromatography , biochemistry , chemistry , genetics
H olton , R aymond W. (U. Texas, Austin.) Isolation, growth, and respiration of a thermophilic blue‐green alga. Amer. Jour. Bot. 49(1): 1–6. Illus. 1962.—The isolation of a bacteria‐free culture of Hapalosiphon laminosus (= Mastigocladus laminosus ) from an algal mass obtained from Liard Hot Springs, British Columbia, is described. Seven different bacterial media at 2 incubation temperatures were used to test for bacteria in suspensions of partially broken up algae. The algal growth rate was obtained by calculating the slope of the straight line obtained in a plot of the cube root of the dry wt against time. In cultures aerated with air the growth was optimal between 35 and 50 C, with an upper limit at 55 and a lower limit at 25. Aeration with 1% CO 2 in air significantly increased growth rates and the optimum was at 45–50 C. Unaerated flasks grew more slowly than aerated ones. The endogenous Qo 2 (in μl/hr–mg dry wt) at 45 was 8.0 for growing cells and 2.8 for starved cells kept in darkness 18 hr. The Qo 2 was maximal between pH 7.0 and 8.5. The concentration of phosphate buffer affected the Qo 2 and rates in 0.02 M were slightly greater than those in distilled water and much greater than those in 0.4 M. The Qo 2 of growing cells was slightly stimulated by 2,4‐dinitrophenol (DNP), whereas glucose or glucose plus DNP did not affect it. In contrast, the endogenous Qo 2 of starved cells was nearly doubled in glucose and tripled in DNP plus glucose.