Premium
HETEROCARYON INCOMPATIBILITY IN NEUROSPORA CRASSA—MICRO‐INJECTION STUDIES
Author(s) -
Wilson James F.,
Garnjobst Laura,
Tatum Edward L.
Publication year - 1961
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1961.tb11641.x
Subject(s) - neurospora crassa , neurospora , cytoplasm , biology , crassa , gene , biochemistry , genetics , microbiology and biotechnology , mutant
W ilson , J ames F. (Hartnell Coll., Salinas, Calif.), L aura G arnjobst , and E dward L. T atum . Heterocaryon incompatibility in Neurospora crassa—micro‐injection studies. Amer. Jour. Bot. 48(4): 299–305. 1961.—Heterocaryosis in Neurospora crassa is known to be controlled, at least in part, by the genes CD and their alternate alleles cd. If either or both genes differ, a lethal, cytoplasmic incompatibility reaction occurs, effectively preventing the formation of a heterocaryon between strains of the same mating type. This reaction has been further investigated, utilizing a micrurgical approach involving micro‐injection of cytoplasm and extracts. The injection of compatible cytoplasm and extracts was harmless to the recipient cells, but injection of incompatible cytoplasm and extracts was lethal. The extracts were subjected to various chemical and physical treatments and tested, using micro‐injection as an assay method. The results of these tests strongly indicate that a soluble cytoplasmic protein (s) is involved in the heterocaryon‐incompatibility reaction.