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DURATION OF THE NUCLEAR CYC LE IN TRADESCANTIA PALUDOSA ROOT TIPS AS MEASURED WITH H 3 ‐THYMIDINE
Author(s) -
Wimber Donald E.
Publication year - 1960
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1960.tb07171.x
Subject(s) - tradescantia , interphase , telophase , biology , prophase , metaphase , thymidine , anaphase , mitosis , botany , cell cycle , microbiology and biotechnology , horticulture , dna , genetics , chromosome , meiosis , cell , gene
W imber , D onald E. (Brookhaven National Lab., Upton, N. Y.) Duration of the nuclear cycle in Tradescantia paludosa root tips as measured with H 3 ‐thymidine. Amer. Jour. Bot. 47(10): 828–834. Illus. 1960.—The duration of the nuclear cycle and its various subdivisions were measured in Tradescantia root tips by autoradiographic techniques. H 3 ‐thymidine was used as a nuclear label and was supplied to the roots for 0.5 hr. After labeling, the roots were allowed to grow in the absence of label for periods up to 38 hr. By determining the percentage of divisions labeled at the various times of fixation, a reconstruction of the nuclear cycle could be made. The average cycle was determined as 20 hr. in duration, DNA synthesis 10.8 hr., presynthetic interphase 4 hr., postsynthetic interphase 2.7 hr., prophase 1.6 hr., metaphase 0.3 hr. and anaphase‐telophase 0.6 hr. Approximate standard deviations for the duration of some of the subdivisions were calculated.