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CATABOLISM OF C 14 ‐LABELLED GLUCOSE, GLUCONATE AND ACETATE IN VERTICILLIUM ALBO‐ATRUM
Author(s) -
Brandt W. H.,
Wang C. H.
Publication year - 1960
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1960.tb07093.x
Subject(s) - catabolism , biology , verticillium , decarboxylation , biochemistry , glycolysis , metabolism , botany , catalysis
B randt , W. H., and C. H. W ang . (Oregon State College, Corvallis.) Catabolism of C 14 ‐labelled glucose, gluconate and acetate in Vertieillium albo‐atrum . Amer. Jour. Bot. 47(1): 50—53. Illus. 1960.–The major catabolic pathways of glucose in conidia from rapidly proliferating shake‐cultures of Verticillium albo‐atrum Reinke and Berth, isolated from okra and cultured under light at 25°C. have been identified and estimated from data obtained by the radiorespirometric method. When pure oxygen was used as a sweep gas, glucose appeared to be catabolized approximately 60% via the Embden‐Meyerhof‐Parnas glycolysis (EMP) and 40% via phosphogluconate decarboxylation (PGD). With air as the sweep gas, about 48% of the glucose was catabolized via the EMP pathway and about 52% via the PGD pathway. Glucose was catabolized under a pure nitrogen atmosphere, but there was little PGD, operating under these conditions. Gluconate was rapidly decarboxylated in the C‐l position, but only a small proportion of carbon atoms from other positions appeared in the respiratory CO 2 . Acetate was utilized readily in such a way that the operation of the tri‐carboxylic acid cycle may be inferred. Pyruvate was used slowly because it appeared to enter the conidia slowly.