Premium
Inhibition of E2F‐mediated transcription by p202.
Author(s) -
Choubey D.,
Li S. J.,
Datta B.,
Gutterman J. U.,
Lengyel P.
Publication year - 1996
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1996.tb00951.x
Subject(s) - biology , transcription (linguistics) , e2f , transcription factor , genetics , microbiology and biotechnology , computational biology , gene , philosophy , linguistics
Many of the antimicrobial, immunomodulatory and cell growth inhibitory activities of the interferons are mediated by interferon‐inducible proteins. Earlier we characterized an interferon‐inducible murine protein, p202, whose expression in transfected cells inhibits cell proliferation and which can form a complex with retinoblastoma protein (pRb). Here we report that in transfected cells expression of p202 inhibits E2F‐stimulated transcription of a reporter gene and of endogenous genes. Inhibition of the transcriptional activity of E2F by p202 does not depend on fully functional pRb and is correlated with inhibition of the sequence‐specific DNA binding of E2F. p202 interacts with the transcription factor E2F (E2F‐1/DP‐1) in vitro and in vivo. Inhibition of E2F activity by p202 may contribute to growth inhibition by the interferons.