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Ca2+ triggers massive exocytosis in Chinese hamster ovary cells.
Author(s) -
Coorssen J. R.,
Schmitt H.,
Almers W.
Publication year - 1996
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1996.tb00752.x
Subject(s) - exocytosis , chinese hamster ovary cell , gtpgammas , biology , microbiology and biotechnology , cytosol , biophysics , cell culture , secretion , endocrinology , biochemistry , signal transduction , g protein , enzyme , genetics
We have tracked the cell surface area of CHO cells by measuring the membrane capacitance, Cm. An increase in cytosolic [Ca2+], [Ca2+]i, increased the cell surface area by 20–30%. At micromolar [Ca2+]i the increase occurred in minutes, while at 20 microM or higher [Ca2+]i it occurred in seconds and was transient. GTPgammaS caused a 3% increase even at 0.1 microM [Ca2+]i. We conclude that CHO cells, previously thought capable only of constitutive exocytosis, can perform Ca2+‐triggered exocytosis that is both massive and rapid. Ca2+‐triggered exocytosis was also observed in 3T3 fibroblasts. Our findings add evidence to the view that Ca induces exocytosis in cells other than known secretory cells.