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Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage.
Author(s) -
Ramsden D. A.,
McBlane J. F.,
Gent D. C.,
Gellert M.
Publication year - 1996
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1996.tb00682.x
Subject(s) - biology , recombination , cleavage (geology) , dna , sequence (biology) , genetics , microbiology and biotechnology , computational biology , biophysics , gene , paleontology , fracture (geology)
Cleavage of V(D)J recombination signals by purified RAG1 and RAG2 proteins permits the dissection of DNA structure and sequence requirements. The two recognition elements of a signal (nonamer and heptamer) are used differently, and their cooperation depends on correct helical phasing. The nonamer is most important for initial binding, while efficient nicking and hairpin formation require the heptamer sequence. Both nicking and hairpin formation are remarkably tolerant of variations in DNA structure. Certain flanking sequences inhibit hairpin formation, but this can be bypassed by base unpairing, and even a completely single‐stranded signal sequence is well utilized. We suggest that DNA unpairing around the signal‐coding border is essential for the initiation of V(D)J combination.