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MAT1, cdk7 and cyclin H form a kinase complex which is UV light‐sensitive upon association with TFIIH.
Author(s) -
Adamczewski J. P.,
Rossignol M.,
Tassan J. P.,
Nigg E. A.,
Moncollin V.,
Egly J. M.
Publication year - 1996
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1996.tb00538.x
Subject(s) - physics , microbiology and biotechnology , cyclin dependent kinase 7 , biology , transcription factor ii h , kinase , genetics , gene , gene expression , protein kinase a , map kinase kinase kinase , promoter
MAT1, cyclin H and cdk7 are part of TFIIH, a class II transcription factor which possesses numerous subunits of which several have been shown to be involved in processes other than transcription. Two of them, XPD (ERCC2) and XPB (ERCC3), are helicases involved in nucleotide excision repair (NER), whereas cdk7, cyclin H and MAT1 are thought to participate in cell cycle regulation. MAT1, cyclin H and cdk7 exist as a ternary complex either free or associated with TFIIH from which the latter can be dissociated at high salt concentration. MAT1 is strongly associated with cdk7 and cyclin H. Although not strictly required for the formation and activity of the complex, it stimulates its kinase activity. The kinase activity of TFIIH, which is constant during the cell cycle, is reduced after UV light irradiation.