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The transition of pre‐BI to pre‐BII cells is dependent on the VH structure of the mu/surrogate L chain receptor.
Author(s) -
Ye J.,
McCray S. K.,
Clarke S. H.
Publication year - 1996
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1996.tb00496.x
Subject(s) - biology , transition (genetics) , chain (unit) , biophysics , microbiology and biotechnology , computational biology , genetics , physics , gene , astronomy
We have demonstrated previously that the majority (> 90%) of VH12 B cells are absent from the adult peripheral repertoire, and that most that remain have the fourth position at the D‐J function (designated 10/G4). We report here that most VH 12‐expressing pre‐B cells are lost during the transition from the pre‐BI to the pre‐BII cell stage in normal mice, and that pre‐BII cell productive (P) rearrangements ar enriched in 10/G4 CDR3. This coincides with the initial expression of H chain and the generation of the mu/surrogate L chain (SL) receptor. In contrast, there is not enrichment for 10/G4 CDR3 in mu MT mice, and the frequency of P rearrangements is as expected from a random rearrangement mechanism, ruling out a biased rearrangement mechanism unique to VH12. We have also demonstrated that non‐10/G4 mu chains can associate with SL and be expressed on the cell surface, suggesting that they are available on the cell surface for selection. Thus, transition of pre‐BI to pre‐BII cells is dependent on the structure of the VH domain.