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Reading two bases twice: mammalian antizyme frameshifting in yeast.
Author(s) -
Matsufuji S.,
Matsufuji T.,
Wills N. M.,
Gesteland R. F.,
Atkins J. F.
Publication year - 1996
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1996.tb00478.x
Subject(s) - salt lake , library science , biology , computer science , paleontology , structural basin
Programmed translational frameshifting is essential for the expression of mammalian ornithine decarboxylase antizyme, a protein involved in the regulation of intracellular polyamines. A cassette containing antizyme frameshift signals is found to direct high‐level (16%) frameshifting in yeast, Saccharomyces cerevisiae. In contrast to +1 frameshifting in the mammalian system, in yeast the same frame is reached by −2 frameshifting. Two bases are read twice. The −2 frameshifting is likely to be mediated by slippage of mRNA and re‐pairing with the tRNA in the P‐site. The downstream pseudoknot stimulates frameshifting by 30‐fold compared with 2.5‐fold in reticulocyte lysates. When the length of the spacer between the shift site and the pseudoknot is extended by three nucleotides, +1 and −2 frameshifting become equal.