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The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T‐DNA into the plant genome.
Author(s) -
Tinland B.,
Schoumacher F.,
Gloeckler V.,
BravoAngel A.M.,
Hohn B.
Publication year - 1995
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1995.tb07364.x
Subject(s) - agrobacterium tumefaciens , biology , humanities , philosophy , genetics , gene , transgene
The VirD2 protein of Agrobacterium tumefaciens was shown to pilot T‐DNA during its transfer to the plant cell nucleus. We analyze here its participation in the integration of T‐DNA by using a virD2 mutant. This mutation reduces the efficiency of T‐DNA transfer, but the efficiency of integration of T‐DNA per se is unaffected. Southern and sequence analyses of integration events obtained with the mutated VirD2 protein revealed an aberrant pattern of integration. These results indicate that the wild‐type VirD2 protein participates in ligation of the 5′‐end of the T‐strand to plant DNA and that this ligation step is not rate limiting for T‐DNA integration.