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The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid‐ and alkaline‐expressed genes by ambient pH.
Author(s) -
Tilburn J.,
Sarkar S.,
Widdick D.A.,
Espeso E.A.,
Orejas M.,
Mungroo J.,
Peñalva M.A.,
Arst H.N.
Publication year - 1995
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1995.tb07056.x
Subject(s) - zinc finger , aspergillus nidulans , biology , transcription factor , gene , zinc finger transcription factor , residue (chemistry) , biochemistry , dna , transcription (linguistics) , linguistics , philosophy , mutant
The pH regulation of gene expression in Aspergillus nidulans is mediated by pacC, whose 678 residue‐derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc mutations mimicking growth at alkaline pH remove between 100 and 214 C‐terminal residues, including a highly acidic region containing an acidic glutamine repeat. Nine pacC+/‐ mutations mimicking acidic growth conditions remove between 299 and 505 C‐terminal residues. Deletion of the entire pacC coding region mimics acidity but leads additionally to poor growth and conidiation. A PacC fusion protein binds DNA with the core consensus GCCARG. At alkaline ambient pH, PacC activates transcription of alkaline‐expressed genes (including pacC itself) and represses transcription of acid‐expressed genes. pacCc mutations obviate the need for pH signal transduction.