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Suppression of villin expression by antisense RNA impairs brush border assembly in polarized epithelial intestinal cells.
Author(s) -
Costa de Beauregard M.A.,
Pringault E.,
Robine S.,
Louvard D.
Publication year - 1995
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1995.tb07017.x
Subject(s) - biology , villin , brush border , brush , rna , antisense rna , microbiology and biotechnology , gene expression , messenger rna , circular rna , sense (electronics) , gene , genetics , actin , membrane , vesicle , engineering , electrical engineering
We have used an antisense RNA strategy to investigate the role of the actin‐associated protein, villin, in the brush‐border morphogenesis of human intestinal CaCO2 cells. Stable expression of a cDNA encoding antisense villin RNA resulted in the permanent down‐regulation of the endogenous villin message and dramatically affected brush‐border assembly. Ultrastructural and immunolocalization studies revealed that epithelial cell polarity was largely maintained. However, in contrast to brush‐border markers such as dipeptidyl‐peptidase IV, the apical localization of sucrase‐isomaltase was specifically impaired. Retransfection of the villin antisense‐expressing cell line with a cDNA encoding a partial sense villin RNA restored both brush‐border assembly and sucrase‐isomaltase apical expression. The suggestion that brush‐border morphogenesis may be important for the trafficking of certain proteins is discussed.