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Apoptosis by a cytosolic extract from Fas‐activated cells.
Author(s) -
Enari M.,
Hase A.,
Nagata S.
Publication year - 1995
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1995.tb00204.x
Subject(s) - apoptosis , fas ligand , biology , cytosol , microbiology and biotechnology , fas receptor , cell culture , programmed cell death , biochemistry , enzyme , genetics
Fas is a type I membrane protein and its activation by binding of the Fas ligand or an agonistic anti‐Fas antibody induces apoptosis in Fas‐bearing cells. In this report we prepared lysates from cells treated with anti‐Fas antibody. The lysates induced apoptotic morphological changes in nuclei from normal mouse liver, accompanied by DNA degradation. The apoptosis‐inducing activity was quickly generated in cells by anti‐Fas antibody and was found in the soluble cytosolic fraction. Induction of the activity in cells was inhibited by a tetrapeptide, acetyl‐Tyr‐Val‐Ala‐Asp‐chloromethylketone, a specific inhibitor of interleukin‐1 beta converting enzyme. Addition of COS cell lysates containing Bcl‐2 to the assay significantly inhibited the apoptotic process, indicating that the in vitro process reflected apoptosis that occurs in intact cells.