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A surrogate 15 kDa JC kappa protein is expressed in combination with mu heavy chain by human B cell precursors.
Author(s) -
Francés V.,
PandrauGarcia D.,
Guret C.,
Ho S.,
Wang Z.,
Duvert V.,
Saeland S.,
MartinezValdez H.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06939.x
Subject(s) - plough , garcia , library science , biology , humanities , philosophy , computer science , agronomy
A novel kappa protein, encoded by a germline JC kappa transcript, is expressed by normal and leukemic human B cell precursors. The transcript displays an open reading frame initiated by a non‐AUG codon, and predicts a 15 kDa molecule which could be readily confirmed by in vitro translation. Cellular expression was demonstrated by immunofluorescence, precipitation and Western blotting. Furthermore, 2‐D gel electrophoresis revealed that germline JC kappa can covalently associate with mu heavy chain at the surface of pre‐B cells. We therefore propose that during B cell lymphopoiesis, two alternative pathways could be operative in which mu heavy chain can either associate with lambda 5 or germ‐line JC kappa.