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Preferential selection of adenosines for modification by double‐stranded RNA adenosine deaminase.
Author(s) -
Polson A.G.,
Bass B.L.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06908.x
Subject(s) - adenosine deaminase , rna , ribonuclease , biology , salt lake , adenosine , biochemistry , gene , paleontology , structural basin
Double‐stranded RNA adenosine deaminase (dsRAD), previously called the double‐stranded RNA (dsRNA) unwinding/modifying activity, modifies adenosines to inosines within dsRNA. We used ribonuclease U2 and a mutant of ribonuclease T1 to map the sites of modification in several RNA duplexes. We found that dsRAD had a 5′ neighbor preference (A = U > C > G) but no apparent 3′ neighbor preference. Further, the proximity of the strand termini affected whether an adenosine was modified. Most importantly, dsRAD exhibited selectivity, modifying a minimal number of adenosines in short dsRNAs. Our results suggest that the specific editing of glutamate receptor subunit B mRNA could be performed in vivo by dsRAD without the aid of specificity factors, and support the hypothesis that dsRAD is responsible for hypermutations in certain RNA viruses.