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The Oct‐1 POU domain stimulates adenovirus DNA replication by a direct interaction between the viral precursor terminal protein‐DNA polymerase complex and the POU homeodomain.
Author(s) -
Coenjaerts F.E.,
Oosterhout J.A.,
Vliet P.C.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06875.x
Subject(s) - pou domain , dna replication , biology , dna , homeobox , genetics , microbiology and biotechnology , gene , transcription factor
The bipartite POU domain of transcription factor Oct‐1 stimulates adenovirus DNA replication through an interaction with the octamer sequence present in the auxiliary origin. Employing an immobilized in vitro DNA replication system, we show that the POU domain enhances the formation of a pre‐initiation complex composed of the viral precursor terminal protein‐DNA polymerase (pTP‐pol) complex and the origin. To investigate the mechanism of stimulation we have explored protein‐protein interactions between the POU domain and the pTP‐pol complex. Such an interaction could be detected using a GST‐POU fusion protein bound to glutathione‐agarose beads. Binding was also observed with the POU homeodomain (POUHD), albeit weaker than with the intact POU domain, but not with the POU specific subdomain. Four point mutations localized in the POUHD were analyzed for pTP‐pol binding. Two of these, E22A and E30A, bound pTP‐pol equally as well as the wild‐type, while the other two, Q24A and E29A, were able to bind 2‐ to 4‐fold better. These mutations are localized in the same region where the HSV transactivator VP16 binds, but did not coincide with the VP16 contacts. A direct correlation between pTP‐pol binding and stimulation of DNA replication in vitro was observed for all mutants, suggesting that stimulation by the POU domain is caused by an interaction with the viral pTP‐pol complex.