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The functional subunit of a dimeric transcription activator protein depends on promoter architecture.
Author(s) -
Zhou Y.,
Pendergrast P.S.,
Bell A.,
Williams R.,
Busby S.,
Ebright R.H.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06776.x
Subject(s) - biology , protein subunit , transcription (linguistics) , activator (genetics) , promoter , transcription factor , genetics , microbiology and biotechnology , gene , gene expression , linguistics , philosophy
In Class I CAP‐dependent promoters, the DNA site for CAP is located upstream of the DNA site for RNA polymerase. In Class II CAP‐dependent promoters, the DNA site for CAP overlaps the DNA site for RNA polymerase, replacing the ‐35 site. We have used an ‘oriented heterodimers’ approach to identify the functional subunit of CAP at two Class I promoters having different distances between the DNA sites for CAP and RNA polymerase [CC(‐61.5) and CC(‐72.5)] and at one Class II promoter [CC(‐41.5)]. Our results indicate that transcription activation at Class I promoters, irrespective of the distance between the DNA sites for CAP and RNA polymerase, requires the activating region of the promoter‐proximal subunit of CAP. In striking contrast, our results indicate that transcription activation at Class II promoters requires the activating region of the promoter‐distal subunit of CAP.