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Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe.
Author(s) -
Dubey D.D.,
Zhu J.,
Carlson D.L.,
Sharma K.,
Huberman J.A.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06671.x
Subject(s) - schizosaccharomyces pombe , biology , schizosaccharomyces , origin of replication , replicon , genetics , plasmid , dna replication , gene , microbiology and biotechnology , saccharomyces cerevisiae
The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are concentrated near three ARS elements (stretches of DNA which permit autonomous plasmid replication). To determine the roles of these ARS elements in the function of the ura4 origin region, we deleted either one or two of them from the chromosome and then assessed the consequences of the deletions by 2D gel electrophoresis. The results suggest that each of the three ARS elements is responsible for the initiation events in its vicinity and that the ARS elements interfere with each other in a hierarchical fashion. It is possible that the large initiation zones of animal cells are similarly composed of multiple mutually interfering origins.