Premium
The calmodulin‐dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans.
Author(s) -
Rasmussen C.,
Garen C.,
Brining S.,
Kincaid R.L.,
Means R.L.,
Means A.R.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06544.x
Subject(s) - biology , aspergillus nidulans , calcineurin , calmodulin , protein subunit , gene , phosphatase , protein phosphatase 2 , calmodulin binding proteins , fungal protein , biochemistry , protein phosphatase 1 , genetics , enzyme , peptide sequence , medicine , surgery , mutant , transplantation
The gene encoding the homologue of the catalytic subunit of the Ca2+/calmodulin‐regulated protein phosphatase 2B (calcineurin A) has been isolated from Aspergillus nidulans. This gene, cnaA+, is essential in this fungal system. Analysis of growth‐arrested cells following gene disruption by homologous recombination reveals that they are blocked early in the cell cycle. The cnaA+ gene encodes a 2.5 kb mRNA and the deduced protein sequence is highly homologous to the calcineurin A subunit of other species. The mRNA varies in a cell cycle‐dependent manner with maximal levels found early in G1 and considerably before the G1/S boundary. As calmodulin is also essential for A.nidulans cell cycle progression and levels rise before the G1/S boundary, our data suggest that calcineurin may represent a primary target for calmodulin at this cell cycle transition point.