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The basis for germline specificity of the hobo transposable element in Drosophila melanogaster.
Author(s) -
Calvi B.R.,
Gelbart W.M.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06427.x
Subject(s) - biology , drosophila melanogaster , transposable element , germline , genetics , drosophilidae , dna transposable elements , drosophila (subgenus) , genome , gene
Previous results suggested that the hobo transposable element is active predominantly in the germline of Drosophila. We investigate germline restriction of hobo transposition by testing in vitro modified elements for their ability to mobilize marked elements in vivo. Although intact hobo elements are germline specific, an hsp70 promoter‐hobo transposase fusion is active in the soma. Analysis of the hsp70‐promoted transcript does not provide evidence for splicing. Moreover, the hobo promoter confers germline bias to a highly sensitive reporter, delta 2‐3 P transposase. These results indicate that hobo transposition is germline specific due to regulation of transposase production at the level of transcription. Thus, although hobo is similar to the P transposable element in organization and tissue specificity, it differs in the underlying mechanism governing germline specific activity.