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STF‐IL‐4: a novel IL‐4‐induced signal transducing factor.
Author(s) -
Schindler C.,
Kashleva H.,
Pernis A.,
Pine R.,
Rothman P.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06388.x
Subject(s) - biology , signal (programming language) , microbiology and biotechnology , genetics , computer science , programming language
The mechanism by which interleukin‐4 (IL‐4) regulates the expression of particular genes is unknown. We have determined that IL‐4 induces a DNA binding factor (termed STF‐IL‐4) which has a strong affinity for an IFN‐gamma activation site (GAS). Interestingly, STF‐IL‐4 also binds to the IL‐4 responsive promoter for the Ig heavy chain germline epsilon transcript. The IL‐4 dependent activation of STF‐IL‐4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF‐IL‐4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon‐induced transcription factors. STF‐IL‐4 appears to be a new member of a growing family of cytokine‐induced transcriptional regulators.