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Antibacterial and haemolytic peptides containing D‐alloisoleucine from the skin of Bombina variegata.
Author(s) -
Mignogna G.,
Simmaco M.,
Kreil G.,
Barra D.
Publication year - 1993
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1993.tb06172.x
Subject(s) - biology , antibacterial peptide , antibacterial activity , biochemistry , microbiology and biotechnology , bacteria , genetics
A family of bombinin‐related peptides is present in the skin of Bombina variegata. These peptides contain 27 residues with Gly as N‐terminus and display antimicrobial activity. From sequence analysis of the cDNAs encoding for the corresponding peptide precursors, the presence of a novel 20‐residue peptide with Ile as N‐terminus was predicted. We have now purified a family of hydrophobic peptides named H1‐H5, whose sequences correspond to the predicted peptide with some variability in positions 1, 2 and 8. In particular, H3‐H5 contain a D‐alloisoleucine residue in the second position. All these peptides display antibacterial and haemolytic activity.