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SAR‐dependent mobilization of histone H1 by HMG‐I/Y in vitro: HMG‐I/Y is enriched in H1‐depleted chromatin.
Author(s) -
Zhao K.,
Käs E.,
Gonzalez E.,
Laemmli U.K.
Publication year - 1993
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1993.tb05993.x
Subject(s) - biology , library science , histone , microbiology and biotechnology , genetics , computer science , gene
An experimental assay was developed to search for proteins capable of antagonizing histone H1‐mediated general repression of transcription. T7 RNA polymerase templates containing an upstream scaffold‐associated region (SAR) were highly selectively repressed by H1 relative to non‐SAR control templates. This is due to the nucleation of H1 assembly into flanking DNA brought about by the numerous A‐tracts (AT‐rich sequences containing short homopolymeric runs of dA.dT base pairs) of the SAR. Partial, selective titration of these A‐tracts by the high mobility group (HMG) protein HMG‐I/Y led to the complete derepression of transcription from the SAR template by inducing the redistribution of H1 on to non‐SAR templates. SARs are associated with many highly transcribed regulated genes where they may serve to facilitate the HMG‐I/Y‐mediated displacement of histone H1 in chromatin. Indeed, HMG‐I/Y was found to be strongly enriched in the H1‐depleted subfraction which can be isolated from chromatin.