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A novel gene product associated with mu chains in immature B cells.
Author(s) -
Shirasawa T.,
Ohnishi K.,
Hagiwara S.,
Shigemoto K.,
Takebe Y.,
Rajewsky K.,
Takemori T.
Publication year - 1993
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1993.tb05831.x
Subject(s) - biology , gene , genetics , gene product , product (mathematics) , microbiology and biotechnology , gene expression , geometry , mathematics
A previously unreported B cell specific gene, which we have named 8HS‐20, was isolated from the cDNA library of a pre‐B cell clone by subtraction and differential hybridization. This gene is selectively expressed as a 0.75 kb transcript in pre‐B and bone marrow‐derived B cell lines; a transcript of the same size is also found in bone marrow and, albeit at low levels, in spleen. The deduced amino acid sequence of the 8HS‐20 cDNA displayed homology to a B cell specific gene, VpreB‐1, and to members of the immunoglobulin supergene family including V lambda, V kappa, VH, TCRV alpha, V beta and CD8. Biochemical analysis using purified antiserum against 8HS‐20 oligopeptides indicates that the gene encodes proteins with mol. wts of 13.5, 14, 15.5 and 16 kDa, which associate with mu chains in pre‐B cell lines, and that these molecules are expressed concomitantly with VpreB‐1 and lambda 5 gene products in the same cell lines.