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N‐myc suppresses major histocompatibility complex class I gene expression through down‐regulation of the p50 subunit of NF‐kappa B.
Author(s) -
Veer L.J.,
Beijersbergen R.L.,
Bernards R.
Publication year - 1993
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1993.tb05645.x
Subject(s) - biology , molecular genetics , genetics , gene , general hospital , major histocompatibility complex , microbiology and biotechnology , medicine , emergency medicine
In neuroblastoma, N‐myc suppresses the expression of major histocompatibility complex (MHC) Class I antigens by reducing the binding of a nuclear factor to the enhancer‐A element in the MHC Class I gene promoter. We show here that the p50 subunit of NF‐kappa B is part of this complex and that expression of p50 mRNA is suppressed by N‐myc. Transfection of a p50 expression vector in neuroblastoma cells that express N‐myc at a high level leads to restoration of factor binding to the MHC Class I gene enhancer, restores enhancer activity and leads to re‐expression of MHC Class I antigens at the cell surface. These data indicate that the p50 subunit of NF‐kappa B is involved in the regulation of MHC Class I antigen expression and that N‐myc down‐regulates MHC Class I gene expression primarily through suppression of p50 expression.