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Dominant‐negative mutants of a yeast G‐protein beta subunit identify two functional regions involved in pheromone signalling.
Author(s) -
Leberer E.,
Dignard D.,
Hougan L.,
Thomas D.Y.,
Whiteway M.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05586.x
Subject(s) - research council , biology , genetics , library science , political science , government (linguistics) , linguistics , philosophy , computer science
The STE4 gene, which encodes the beta subunit of the mating response G‐protein in the yeast Saccharomyces cerevisiae, was subjected to a saturation mutagenesis using ‘doped’ oligodeoxynucleotides. We employed a genetic screen to select dominant‐negative STE4 mutants, which when overexpressed from the GAL1 promoter, interfered with the signalling function of the wild type protein. The identified inhibitory amino acid alterations define two small regions that are crucially involved in transmitting the mating signal from G beta to downstream components of the signalling pathway. These results underline the positive signalling role of yeast G beta and assign for the first time the positive signalling function of a G‐protein beta subunit to specific structural features.