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An SRY‐related gene expressed during spermatogenesis in the mouse encodes a sequence‐specific DNA‐binding protein.
Author(s) -
Denny P.,
Swift S.,
Connor F.,
Ashworth A.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05455.x
Subject(s) - testis determining factor , biology , genetics , gene , library science , y chromosome , computer science
SRY, the testis determining gene, encodes a member of a family of DNA binding proteins characterized by an amino acid sequence motif known as the HMG box. Using degenerate primers and the polymerase chain reaction, we have isolated SRY‐related cDNAs from adult murine testis RNA. One of these, Sox‐5, encodes a 43 kDa HMG‐box protein with similarities to transcription activating proteins. Anti‐Sox‐5 antibody was used to analyse expression of Sox‐5 in pre‐pubertal testis and in fractionated spermatogenic cells. Sox‐5 is restricted to post‐meiotic germ cells, being found at highest levels in round spermatids. Sox‐5 is a DNA binding protein and binding site selection assays suggest that it can bind specifically to oligonucleotides containing the consensus motif AACAAT. Sry can also bind to this motif, indicating that the Sry family may have overlapping sequence specificities.