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The human poly(ADP‐ribose) polymerase nuclear localization signal is a bipartite element functionally separate from DNA binding and catalytic activity.
Author(s) -
Schreiber V.,
Molinete M.,
Boeuf H.,
Murcia G.,
Ménissierde Murcia J.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05404.x
Subject(s) - biology , poly adp ribose polymerase , polymerase , dna , microbiology and biotechnology , nuclear localization sequence , dna binding protein , biochemistry , transcription factor , gene
Poly(ADP‐ribose) polymerase (PARP, EC 2.4.2.30) is a zinc finger DNA‐binding protein involved in DNA repair processes in eukaryotes. By deletion and extensive site‐directed mutagenesis, its DNA‐binding domain fused to the N‐terminus of beta‐galactosidase was shown to contain a nuclear localization signal (NLS) of the form KRK‐X(11)‐KKKSKK (residues 207–226). In vitro, both the DNA‐binding capacity and the polymerizing activity of PARP are independent of the nuclear location function. Each basic cluster is essential but not sufficient on its own for this function, while both motifs together are. Crucial basic amino acids (K207, R208 and K222) in each of these two motifs are required for nuclear homing. The results presented here support the concept that the human PARP NLS is an autonomous functional element and belongs to the class of bipartite NLSs. We show that the linear distance between the two basic clusters is not crucial. Insertional mutation analysis leading to a partial reversion of the cytoplasmic phenotype displayed by the mutant K222I highlights the crucial positioning of this lysine. The structure‐function relationship of the second cluster of basic residues is discussed.