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Stable and functional expression of the calcium channel alpha 1 subunit from smooth muscle in somatic cell lines.
Author(s) -
Bosse E.,
Bottlender R.,
Kleppisch T.,
Hescheler J.,
Welling A.,
Hofmann F.,
Flockerzi V.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05260.x
Subject(s) - r type calcium channel , biology , chinese hamster ovary cell , calcium channel , t type calcium channel , n type calcium channel , voltage dependent calcium channel , interleukin 10 receptor, alpha subunit , protein subunit , l type calcium channel , g alpha subunit , microbiology and biotechnology , dihydropyridine , calcium , interleukin 5 receptor alpha subunit , myocyte , biochemistry , receptor , medicine , gene
Voltage‐activated calcium channels are membrane spanning proteins that allow the controlled entry of Ca2+ into the cytoplasm of cells. The principal channel forming subunit of an L‐type calcium channel is the alpha 1 subunit. Transfection of Chinese hamster ovary (CHO) cells with complementary DNA encoding the calcium channel alpha 1 subunit from smooth muscle led to the expression of functional calcium channels which bind calcium channel blockers and show the voltage‐dependent activation and slow inactivation and unitary current conductance characteristic of calcium channels in smooth muscle. The currents mediated by these channels are sensitive towards dihydropyridine‐type blockers and agonists indicating that the calcium channel blocker receptor sites were present in functional form. The smooth muscle alpha 1 subunit cDNA alone is sufficient for stable expression of functional calcium channels with the expected kinetic and pharmacological properties in mammalian somatic cells.