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Domain structure of the human immunodeficiency virus reverse transcriptase.
Author(s) -
Lederer H.,
Schatz O.,
May R.,
Crespi H.,
Darlix J.L.,
Le Grice S.F.,
Heumann H.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05153.x
Subject(s) - biology , reverse transcriptase , virology , human immunodeficiency virus (hiv) , rna directed dna polymerase , virus , genetics , rna , gene
The spatial arrangement of subunits p51 and p66 of the HIV‐1 reverse transcriptase and the position of the RNase H containing domain, p15, have been determined by means of neutron small‐angle scattering. The reverse transcriptase (p66/p51) is a flat molecule, which can be approximated by an ellipsoid with the half axes of 5.2 nm, 4.8 nm and 1.4 nm. The two subunits p51 and p66 having a centre‐to‐centre distance of 3.3 +/− 0.3 nm are attached at their flat sides, slightly shifted sideways. The p15 domain is located at the long axis of the ellipsoidal reverse transcriptase having a distance of 5.0 +/− 0.5 nm to the centre of the p51d domain, which is part of the p66 subunit, and a distance of 5.3 +/− 1.2 nm to the centre of the neighbouring p51s subunit.