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Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism.
Author(s) -
Usuda S.,
Takemori T.,
Matsuoka M.,
Shirasawa T.,
Yoshida K.,
Mori A.,
Ishizaka K.,
Sakano H.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05093.x
Subject(s) - biology , dna , immunoglobulin gene , gene , genetics , mechanism (biology) , antibody , microbiology and biotechnology , philosophy , epistemology
Circular DNA resulting from V gene replacement was studied with an A‐MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer‐related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre‐B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer‐like sequence in the Ig H region.