Premium
The TATA box promoter region of maize Adh1 affects its organ‐specific expression.
Author(s) -
KloeckenerGruissem B.,
Vogel J.M.,
Freeling M.
Publication year - 1992
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1992.tb05038.x
Subject(s) - biology , tata box , plant biology , golden gate , computational biology , genetics , gene , promoter , botany , gene expression , engineering , civil engineering , span (engineering)
We have isolated two lineage‐related Mutator (Mu3) transposon‐induced Adh1 promoter mutants in maize: Adh1‐3F1124 carries a duplicated TATA box and its revertant, Adh1‐3F1124r17, bears a deleted TATA box. Both alterations lead to unique patterns of organ‐specific ADH1 enzyme expression. Enzyme activity in Adh1‐3F1124 sporophytic organs (scutellum and roots) is greatly reduced, while activity levels remain normal in the male gametophyte (pollen). Conversely, enzyme activity in Adh1‐3F1124r17 roots and scutellum is partially restored, but is concomitantly reduced in pollen. Transcript analysis suggests (i) that the TATA box region of the Adh1 gene influences post‐transcriptional processes in the male gametophyte but not in roots and (ii) that organ‐specific transcription signals in the promoter are distinct from the previously identified anaerobic environment‐specific cis‐acting transcription signals. Different organs appear to provide surrogate TATA function in different ways, leading to organ‐specific differences in the length of the Adh1 message 5′ leader.