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The c‐Ets oncoprotein activates the stromelysin promoter through the same elements as several non‐nuclear oncoproteins.
Author(s) -
Wasylyk C.,
Gutman A.,
Nicholson R.,
Wasylyk B.
Publication year - 1991
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1991.tb08053.x
Subject(s) - biology , nuclear protein , nuclear transport , cell nucleus , microbiology and biotechnology , cancer research , genetics , transcription factor , gene
The c‐ets protooncogenes have recently been shown to code for transcription factors that activate the oncogene responsive unit of the polyoma virus enhancer. We show that transcription of the stromelysin gene, which is highly expressed in transformed cells and tumours, is efficiently activated by c‐Ets‐1 and ‐2 through two DNA elements. The distal element is a highly conserved palindrome composed of two strong binding sites for c‐Ets‐1. The proximal element does not bind c‐Ets‐1, but may be activated indirectly by increased synthesis of c‐Jun and c‐Fos. Both ets responsive elements mediate activation by the oncoproteins Ha‐Ras, v‐Src and v‐Mos. These results suggest that c‐Ets participates in the mechanisms by which stromelysin gene expression is deregulated in transformed cells and tumours.