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Axonal transport of neuropeptide encoding mRNAs within the hypothalamo‐hypophyseal tract of rats.
Author(s) -
Mohr E.,
Fehr S.,
Richter D.
Publication year - 1991
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1991.tb07781.x
Subject(s) - biology , vasopressin , oxytocin , posterior pituitary , hypothalamus , neuropeptide , in situ hybridization , neurophysins , axoplasmic transport , messenger rna , medicine , endocrinology , pituitary gland , microbiology and biotechnology , gene , genetics , hormone , receptor
Hypothalamic vasopressin and oxytocin transcripts have been detected in the posterior pituitary suggesting either transcription of the respective genes in pituicytes or axonal mRNA transport from the hypothalamus to the nerve terminals of the posterior pituitary. The concept of axonal mRNA transport is supported firstly, by Northern blot and in situ hybridization analysis indicating that vasopressin and oxytocin mRNAs are also present in the neural stalk; secondly, by intron analysis and transcription run on experiments demonstrating the absence of primary vasopressin and oxytocin transcripts in non‐neuronal cells of the posterior pituitary; thirdly, by embryonic developmental studies showing that appearance of vasopressin transcripts in the hypothalamus and the pituitary anlage is correlated. Furthermore, during axonal transport the respective mRNAs are subject to specific modification at the poly(A) tails.