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A specific DNA sequence is required for high frequency of recombination in the ade6 gene of fission yeast.
Author(s) -
Schuchert P.,
Langsford M.,
Käslin E.,
Kohli J.
Publication year - 1991
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1991.tb07750.x
Subject(s) - biology , recombination , genetics , dna , gene , yeast , sequence (biology) , fission , homologous recombination , schizosaccharomyces , schizosaccharomyces pombe , dna sequencing , saccharomyces cerevisiae , physics , quantum mechanics , neutron
The point mutation M26 in the ade6 gene of Schizosaccharomyces pombe increases recombination frequency by an order of magnitude in comparison with other mutations in the same gene. The hypothesis is tested that this hot spot of recombination requires a specific nucleotide sequence at the M26 site. The DNA sequence is altered systematically by in vitro mutagenesis, and the resulting sequences are introduced into the ade6 gene in vivo by gene replacement. It results that any change of the heptanucleotide ATGACGT leads to loss of high frequency of recombination. Thus this oligonucleotide sequence is necessary for high frequency of recombination, but it seems not to be sufficient.