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TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III.
Author(s) -
Simmen K.A.,
Bernués J.,
Parry H.D.,
Stunnenberg H.G.,
Berkenstam A.,
Cavallini B.,
Egly J.M.,
Mattaj I.W.
Publication year - 1991
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1991.tb07711.x
Subject(s) - transcription factor ii d , transcription factor ii a , biology , general transcription factor , taf1 , transcription factor ii b , taf2 , rna polymerase ii , rna polymerase ii holoenzyme , microbiology and biotechnology , transcription factor ii f , transcription (linguistics) , polymerase , rna dependent rna polymerase , genetics , gene expression , promoter , gene , linguistics , philosophy
We present evidence that transcription factor TFIID, known for its central role in transcription by RNA polymerase II, is also involved in RNA polymerase III transcription of the human U6 snRNA gene. Recombinant human TFIID, expressed either via a vaccinia virus vector in HeLa cells or in Escherichia coli, affects U6 transcription in three different in vitro assays. First, TFIID‐containing fractions stimulate U6 transcription in reactions containing rate‐limiting amounts of HeLa nuclear extract. Second, TFIID addition relieves transcriptional exclusion between two competing U6 templates. Third, TFIID can replace one of two heat labile fractions essential for U6 transcription. Thus, at least one basal transcription factor is involved in transcription by two different RNA polymerases.