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Two PDGF‐B chain residues, arginine 27 and isoleucine 30, mediate receptor binding and activation.
Author(s) -
Clements J.M.,
Bawden L.J.,
Bloxidge R.E.,
Catlin G.,
Cook A.L.,
Craig S.,
Drummond A.H.,
Edwards R.M.,
Fallon A.,
Green D.R.
Publication year - 1991
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1991.tb04988.x
Subject(s) - library science , management , operations research , engineering , economics , computer science
PDGF may be involved in the pathogenesis of a variety of disorders including atherosclerosis and certain types of cancer. There is currently little understanding of the molecular structure of PDGF and of the critical amino acid residues involved in receptor binding and cell activation. Two such PDGF‐B chain residues, arginine 27 and isoleucine 30, have been identified by a site‐directed mutagenesis programme. Substitutions in these positions can lead to PDGF mutants defective in both receptor affinity and cell activation as judged by displacement of [125I]PDGF‐BB, mitogenic assay and inositol lipid turnover. Circular dichroism and fluorescence spectroscopy show that such mutations do not disrupt the structure of PDGF.