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The ryh1 gene in the fission yeast Schizosaccharomyces pombe encoding a GTP‐binding protein related to ras, rho and ypt: structure, expression and identification of its human homologue.
Author(s) -
Hengst L.,
Lehmeier T.,
Gallwitz D.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08322.x
Subject(s) - schizosaccharomyces pombe , biology , schizosaccharomyces , gene , yeast , gtp binding protein regulators , genetics , fungal protein , gtp' , dna binding protein , fission , saccharomyces cerevisiae , g protein , biochemistry , enzyme , signal transduction , transcription factor , physics , quantum mechanics , neutron
A gene, ryh1, of the fission yeast Schizosaccharomyces pombe encoding a GTP‐binding protein of 201 amino acids and belonging to the ras superfamily was isolated using the protein‐coding region of the cloned Saccharomyces cerevisiae YPT1 gene as hybridization probe. The ryh1 gene is interrupted by three introns. ryh1 null mutants are viable but unable to grow at temperatures greater than 35.5 degrees C. Invertase of ryh1‐ cells is properly secreted but has a faster electrophoretic mobility compared to that of wild‐type cells. The temperature‐sensitive phenotype of ryh1 null mutants is complemented by the human rab6 cDNA expressed either under transcriptional control of the S.pombe adh or the SV40 early promoter.