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Ectopic expression of c‐jun leads to differentiation of P19 embryonal carcinoma cells.
Author(s) -
Groot R.P.,
Kruyt F.A.,
Saag P.T.,
Kruijer W.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08308.x
Subject(s) - biology , p19 cell , embryonal carcinoma , ectopic expression , c jun , cancer research , cellular differentiation , microbiology and biotechnology , genetics , cell culture , transcription factor , gene , adult stem cell
The product of the c‐jun proto‐oncogene, a major component of the transcription factor AP1, has been implicated in both positive and negative transcriptional control as well as in transformation. The role of c‐jun in early development and differentiation processes, however, is still largely unknown. In this paper we show that ectopic expression of exogenous c‐jun sequences leads to differentiation and loss of the transformed phenotype of P19 embryonal carcinoma (EC) cells. The mixed populations of endoderm‐ and mesoderm‐like cells that were obtained after the introduction of c‐jun morphologically and biochemically resembled P19 cells differentiated in monolayer by retinoic acid (RA). Furthermore, we provide evidence that direct effects of the c‐jun gene product on transcription of the retinoic acid receptor beta (RAR beta) gene may be responsible for the differentiation‐promoting potential of c‐jun.