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Establishment of an interleukin‐5‐dependent subclone from an interleukin‐3‐dependent murine hemopoietic progenitor cell line, LyD9, and its malignant transformation by autocrine secretion of interleukin‐5.
Author(s) -
Tohyama K.,
Lee K.H.,
Tashiro K.,
Kinashi T.,
Honjo T.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08307.x
Subject(s) - biology , autocrine signalling , microbiology and biotechnology , interleukin 3 , progenitor cell , haematopoiesis , cell culture , interleukin , stromal cell , stem cell , cancer research , cytokine , immunology , t cell , genetics , interleukin 21 , immune system
An interleukin‐5 (IL‐5)‐dependent subclone, K‐5, was established from an IL‐3‐dependent murine hemopoietic progenitor cell line by co‐culturing with bone marrow stroma cells. K‐5 cells were induced to differentiate into myeloid lineage cells by co‐culturing with cloned PA6 stroma cells. By co‐culturing with another cloned stroma cell (ST‐2s10), K‐5 cells gave rise to a factor‐independent transformant cell line LT‐5 which proliferated in an autocrine manner by secretion of IL‐5 and produced tumors in nude mice. Molecular cloning of the IL‐5 gene of LT‐5 cells and the nucleotide sequencing of its 5′ flanking region indicate that a transposition of an intracisternal A‐particle (IAP) element to the 5′ flanking region of the IL‐5 gene is responsible for the constitutive expression of IL‐5 mRNA of an aberrant size in LT‐5 cells.