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The plant G box promoter sequence activates transcription in Saccharomyces cerevisiae and is bound in vitro by a yeast activity similar to GBF, the plant G box binding factor.
Author(s) -
Donald R.G.,
Schindler U.,
Batschauer A.,
Cashmore A.R.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08296.x
Subject(s) - biology , saccharomyces cerevisiae , yeast , transcription factor , promoter , genetics , dna binding protein , binding site , microbiology and biotechnology , gene , gene expression
G box and I box sequences of the Arabidopsis thaliana ribulose‐bisphosphate‐1,5‐carboxylase small subunit (RBCS) promoter are required for expression mediated by the Arabidopsis rbcS‐1A promoter in transgenic tobacco plants and are bound in vitro by factors from plant nuclear extracts termed GBF and GA‐1, respectively. We show here that a ‐390 to ‐60 rbcS‐1A promoter fragment containing the G box and two I boxes activates transcription from a truncated iso‐1‐cytochrome c (CYC1) gene promoter in Saccharomyces cerevisiae. Mutagenesis of either the rbcS‐1A G box or both I box sequences eliminated the expression mediated by this fragment. When polymerized, I box oligonucleotides were also capable of enhancing expression from the truncated CYC1 promoter. Single‐copy G box sequences from the Arabidopsis rbcS‐1A, Arabidopsis Adh and tomato rbcS‐3A promoters were more potent activators and were used in mobility shift assays to identify a DNA binding activity in yeast functionally similar to GBF. In methylation interference experiments, the binding specificity of the yeast protein was indistinguishable from that obtained with plant nuclear extracts.