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Electron microscopy mapping of pBR322 DNA curvature. Comparison with theoretical models.
Author(s) -
Muzard G.,
Théveny B.,
Révet B.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08238.x
Subject(s) - biology , curvature , dna , electron microscope , pbr322 , computational biology , microscopy , biophysics , genetics , plasmid , physics , optics , geometry , mathematics
A map of local curvature of the pBR322 DNA has been established by electron microscopy analysis of linearized plasmid molecules. To determine their polarity these molecules are one end labelled with an avidin‐ferritin‐biotin complex and the images are digitized. Local curvature is calculated from two mathematical treatments of the DNA trajectory and expressed in term of a mean dinucleotide wedge angle. Eight regions of curvature are distinguished. The four main regions of curvature have a high content of phased AA runs. The experimental curvature map is compared to theoretical maps of curvature obtained from four available models for DNA curvature.