Premium
Mutations affecting primer RNA interaction with the replication repressor RNA I in plasmid CoIE1: potential RNA folding pathway mutants.
Author(s) -
Polisky B.,
Zhang X.Y.,
Fitzwater T.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08108.x
Subject(s) - biology , rna , plasmid , genetics , pseudoknot , repressor , mutant , cole1 , riboswitch , microbiology and biotechnology , non coding rna , gene , gene expression
The control of plasmid ColE1 copy number is mediated by the kinetics of interaction of two complementary plasmid‐encoded RNAs. One RNA is the primer precursor and the other is a small counter‐transcript called RNA I. The interaction of these highly structured RNAs results in inhibition of formation of mature primer RNA necessary for replication initiation. We have studied several plasmid copy number mutants which have single base changes in the primer which render the primer resistant to inhibition by RNA I despite the fact that the mutations are located outside the overlap between primer and RNA I. We propose a model to account for the resistance of the mutant primers which is based on the differential folding of the nascent primer transcripts during transcription. We propose that the mutant primers diverge in structure from their wild‐type counterparts during a discrete period during transcription. During this brief divergence, they are proposed to interact kinetically more slowly with RNA I than wild‐type primer because a particular domain (the anti‐tail) required for efficient interaction with RNA I is buried in a stem‐loop structure while this same domain is predicted to be single‐stranded in the wild‐type. Despite substantial sequence divergence from ColE1, the primer precursors of the related plasmids CloDF13, RSF1030 and p15A also have retained the potential to expose their anti‐tail in a similar manner to ColE1, suggesting that the folding pathway has been conserved in evolution.(ABSTRACT TRUNCATED AT 250 WORDS)