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Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.
Author(s) -
Bindereif A.,
Wolff T.,
Green M.R.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08102.x
Subject(s) - snrnp , biology , rna splicing , small nuclear rna , prp24 , spliceosome , computational biology , genetics , ribonucleoprotein , microbiology and biotechnology , rna , gene , non coding rna
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.