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Identification of sequences responsible for positive and negative regulation by E1A in the promoter of H‐2Kbm1 class I MHC gene.
Author(s) -
Katoh S.,
Ozawa K.,
Kondoh S.,
Soeda E.,
Israel A.,
Shiroki K.,
Fujinaga K.,
Itakura K.,
Gachelin G.,
Yokoyama K.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb08088.x
Subject(s) - biology , genetics , gene , mhc class i , identification (biology) , major histocompatibility complex , promoter , computational biology , gene expression , botany
The mechanism of transcriptional regulation of the H‐2Kbm1 major histocompatibility complex (MHC) class I gene by adenovirus type 12 E1A (Ad12‐E1A) was studied in transfected rat embryonal fibroblasts. Results of long‐term expression of the chloramphenicol acetyl transferase (CAT) gene placed under the control of the 5′‐flanking region of the mouse MHC class I gene. H‐2Kbm1, and the results of nuclear run‐on transcription assays, yield evidence for both positive and negative regulation of H‐2Kbm1 by E1A gene product. Deletion studies in the H‐2Kbm1 promoter region revealed that a proximal 58 bp upstream sequence (‐194 to ‐136, relative to the cap site) and a distal 316 bp sequence (‐1837 to ‐1521) respectively contribute to positive and negative regulation mediated by the E1A gene product. Both regulatory elements of MHC class I gene promoter region are responsible for the differential expression of the H‐2Kbm1 gene in Ad12 transformed cells. A nuclear factor binding to the negative element has been detected only in extracts derived from cells expressing Ad12‐E1A.