Premium
Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction.
Author(s) -
Utans U.,
Krämer A.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb07634.x
Subject(s) - rna splicing , biology , sr protein , alternative splicing , splicing factor , ribonucleoprotein , minigene , messenger rna , microbiology and biotechnology , rna , genetics , gene
The splicing of nuclear messenger RNA precursors (pre‐mRNA) can be reconstituted in vitro with factors partially purified from HeLa cell nuclear extracts. Splicing complexes are assembled in the presence of the small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5 and U6 and the protein factors SF1, SF2, SF3 and U2AF. However, the complexes thus formed are inactive, i.e. they only contain unprocessed pre‐mRNA. The intermediates and products of the splicing reaction are generated after addition of SF4. This splicing factor is a heat‐labile protein which requires sulfhydryl groups for its activity. SF4 appears to participate, directly or indirectly, in the conversion of a functional but inactive splicing complex to the active spliceosome.